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| Maia,O.B.; Jácomo,A.T.A.; Bringel,B.A.; Kashivakura,C.K.; Oliveira,C.A.; Teodoro,L.O.F.; Silveira,L.; Teixeira da Costa,M.E.L.; Malta,M.C.C.; Furtado,M.M.; Torres,N.M.; Mattos,P.S.R.; Viau,.P; Lima,T.F.G.; Morato,R.G.. |
| Serum hormone levels were compared between captive and free-living maned wolves and seasonal variations of sex hormones were studied. Blood samples were collected from 16 male and 26 female adult animals from Brazilian zoos, and from 30 male and 24 female free-living adults to determine serum progesterone and testosterone by radioimmunoassay. Serum testosterone concentrations varied (P < 0.05) across seasons for 16 captive males, being higher in autumn (2184.7 ± 355.1 pg/mL) than in summer (1080.7 ± 205.4 pg/mL), winter (1270.1 ± 276.6 pg/mL) and spring (963.9 ± 248.1 pg/mL), although they did not differ between summer, winter and spring. Testosterone concentration of 30 free-living males differed (P < 0.05) between autumn (824.1 ± 512.2 pg/mL),... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Maned wolf Chrysocyon brachyurus; Progesterone; Testosterone; Radioimmunoassay; Reproduction. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200015 |
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| Paz,R.C.R.; Souza,N.P.; Furtado,P.V.; Brown,J.L.. |
| ABSTRACT In this study, four crab-eating fox females (Cerdocyon thous) maintained at the Federal University of Mato Grosso Zoo, Cuiabá, Brazil, were investigated for 12 months, using feces measurement of estradiol and progesterone concentrations. Fecal collections were performed three times a week for hormone extraction. Two methods of analysis, Elisa (EIA) and Radioimmunoassay (RIA), were used in the measurement of progesterone (P4) and estradiol (E2) metabolites. The aim of this study was to compare and validate two different methods of hormone measurement for C. thous. There were no differences regarding the method used. The Radioimmunoassay technique proved to be more sensitive, however, both showed similar results. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Radioimmunoassay; Elisa; Estradiol; Progesterone; Fecal. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300636 |
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| Chelini,M.O.M.; Souza,N.L.; Rocha,A.M.; Felippe,E.C.G.; Oliveira,C.A.. |
| Alternative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a non-invasive, non-stressor, economical, and animal saving technique which allows longitudinal studies by permitting frequent sampling of the same individual. The present study was undertaken to determine the suitability of this method for laboratory animals. Estradiol and progesterone metabolites were quantified by radioimmunoassay in feces of intact, sexually mature female Syrian hamsters during the estrous cycle... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endocrinology; Fecal steroids; Syrian hamster; Reproduction; Radioimmunoassay; Estrogen. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100021 |
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